新型耐酸性α-淀粉酶液态深层发酵条件的研究

Study on Fermentation Conditions of Novel Acid-resistant α-amylase in Liquid Culture

本文采用白曲霉基因工程茵株TR12,该菌株是通过基因工程技术导入了产生耐酸性α-淀粉酶和糖化酶的多拷贝融合基因而获得的。通过正交试验的方法对TR12的液态深层发酵条件进行研究,获得了一最佳摇瓶发酵培养基配方A_38_3C_2D_1。为了验证该理论结果,又利用摇瓶和2L的通气发酵罐进行了放大试验。

A genetic engineering strain TR12 Aspergillus Kawachii was used . Fermentation study of TR12 in liquid culture medium A3D3C2D1 was chosen . To test the pure results , we used an aerated - fermentor and did an amplifying experiment .