姬松茸多糖脱蛋白方法的研究

Study on Removal of Protein from polysaccharide of Agaricus Blazei Murill

采用四种方法对姬松茸子实体多糖进行脱蛋白提纯,结果表明:酶法和三氯乙酸-正丁醇法较好,将两种方法结合使用发现,当pH为5时,40℃水浴酶解1h,再加入2倍于多糖溶液体积的三氯乙酸-正丁醇液(V/V=1∶10),振摇15min,静置1.5h,蛋白质去除率最高。

Four schemes were used to remove protein from the polysaccharide of Agaricus Blazei Murill.The results showed schemes of enzyme and Trichloroaceticacid-n-Butanol were better than others.Combining these two schemes,the optimum parameters were obtained.When the polysaccharide solution was enzymic hydrolysis for an hour in 40℃water of pH5.0,added Trichloroaceticacid-n-Butanol twice as much as polysaccharide solution,stired for 15 minutes,and put for one and a half hour,the removal of protein reach the maxium.