黑孢块菌的苗木菌根化合成效果研究

A Study of Mycorrhizal Synthesis Effect of Tuber melanosporum on Seedlings

黑孢块菌菌根化幼苗是该菌在树木上完成半人工模拟栽培的基础。采用黑孢块菌的液体纯菌种1 4mL对4个树种幼苗进行接种,90d后都能形成菌根,其感染率达94.4% 100%;菌根形态主要有4种类型,以珊瑚状居多,其次为棒状及二叉状,但不同树种上其各类型所占比例各不相同;黑孢块菌不同接种量的云南松幼苗,平均高比对照增加76.67% 131.74%,而生物量则增加1.62 2.17倍,以每株接种4mL菌液的效果为佳;不同pH值的基质试验结果表明,pH值为6.5 7.0时其菌根感染率高,生物量增加明显,苗木长势也较好。

Mycorrhizal seedlings are the basis of mycorrhizal synthesis of Tuber melanosporum on the trees roots in the soil. Four species of seedlings were inoculated by using 14 mL liquid inoculum of T.melanosporum. After 90 days, the percentages of mycorrhizal infection were 94.4%100%. The mycorrhizas had four forms, in which the coral form was the most common, followed by the stick and bifurcate forms, while the spherical form was the least. But the proportion of each mycorrhizal form was different on various tree species. After inoculation, the height of seedlings of Pinus yunnanensis  with T. melanosporum was increased by 76.67%131.74%, the mean dry weight was increased by 162%217%. The seedlings with 4 mL liquid inoculum performed better. The results of media experiment with different pH values showed that the pH 6.57.0 had higher inoculation rate and biomass increment as well as better seedling growth.