粘虫胚胎细胞系的建立及对MsNPV敏感性的测定

Establishment of a cell line from embryos of Mythimna separata and its susceptibility to MsNPV

用培养24h的粘虫受精卵成功地建立一株粘虫胚胎细胞后。命名为NEAU-Ms-980312。目前已传代201代,该细胞系主要含有圆形和纺锤形两种细胞,在28℃、120h的细胞群体倍增时间为47.82h。该细胞系对粘虫核型多角体病毒有较高的敏感性,用原代病毒以感染复数(m.o.i.)为0.08TCID50/细胞感染细胞,在接毒后10d,病毒感染率和每细胞产生的多角体数量分别是为62.10％和33.59PIB。

A new cell line, designated NEAU-Ms-980312, was established from 24-hr-old Mythimna separata eggs and was passaged 201 times. The cells were heterogeneous in morphology with spherical and spindle-shaped cells. The population doubling time of the cell line aws 47.82h in 120h at 28℃. The cell line was highly susceptible to Mythimna separata nuclear polyhedrosis virus (MsNPV). When the cells were inoculated at a multiplicity of infection (m.o.i.) of 0.08TCID50/ cell with wild passage MsNPV, the infection rates and occlusion bodies (OB) yield reached a value of 62.10%, 33.59PIB/infected cell at 240h postinfection (pi), respectively.