恒河猴外周血及肾组织SV40病毒基因分析

Genetic Analysis of Simian virus 40 from Peripheral Blood and Kidney of Macaque Monkeys

SV40即猿猴病毒40(simian virus 40),是DNA肿瘤病毒的原型代表,其基因结构为共价闭合环状双股DNA分子,标准参考株SV40-776含5243个核甘酸,不同分离株bp数略有差异.

In order to investigate the detection methods and gene structure of SV40 ST-ag and LT-ag-C in peripheral blood mononuclear cells (PBMC) and kidney cells from different Macaque monkeys, using PCR, 4 positive templates were obtained firstly, then 4 ST-ag gene fragments and 4 LT-ag-C gene fragments were achieved and sequenced. These antigens coding region of each isolate contain a number of nucleotide differences when compared to the reference strain SV40-776 and the laboratory strain SV40-B. These results indicated that; 1) SV40 DNA isolated from the PBMC and kidney cells showed essentially identical sequences for ST-ag gene and LT-ag-C gene. 2) the presence of viral DNA in P-BMC suggests that virus may spread with in the host by hematogenous routes. 3) a new SV40 strain predominates in our monkey population, it contains a specific nucleotide and amino acid changes in LT-ag-C termini, TCCTCACAG polynucleotide is inserted among nt2784/2783, so SerSerGln is inserted after amino acid 678 residue.