山羊β酪蛋白基因克隆及序列分析

Molecular Cloning and Sequence Analysis of Goat β-Casein Gene

用LongPCR技术克隆山羊 β酪蛋白 (CSN2 )全基因 13.7kb的序列 (GenBank登录号 :AF4 0 90 96 ) .利用PCR RFLP方法鉴定第 14和 192位氨基酸残基相应的核苷酸序列中出现的 2个多态性位点 ,其中第 192位氨基酸残基处的TspRⅠ位点是Saanen奶山羊CSN2基因中新发现的 1个多态性位点 .生物信息学方法分析山羊CSN2基因可见 ,非编码区中的SINE和LINE序列内含多种乳蛋白基因表达调控元件 。

The 13 7 kb of the full length sequence of goat β casein(CSN2)gene, accepted by GenBank with Accession Number AF 409096, was cloned by using Long PCR technology. The Bal Ⅰ and Tsp R Ⅰ polymorphic restriction sites, located in the nucleotides encoding for the 14th and 192nd amino acid residues, have been demonstrated by PCR RFLP. Moreover, it was suggested that the Tsp R Ⅰ polymorphic site was a novel allelic type of Saanen goat CSN2 gene. Bioinformatics searching results revealed that short interspersed nuclear element(SINE)as well as long interspersed nuclear element (LINE), situated in non coding regions of the gene, consisted of many clustered regulatory elements of milk protein genes that might be responsible for the expression and post transcriptional control of CSN2 gene during lactogenesis in goat mammary glands.