人卵巢浆液性囊腺癌永生化细胞系BUPH∶OVCA-3的建立及其生物学特性

Establishment and Characterization of an Immortalized Human Ovarian Serous Cystadenocarcinoma Cell Line BUPH∶OVCA-3

介绍人卵巢浆液性囊腺癌永生化细胞系的建立 ,研究其生物学特性 .以卵巢浆液性乳头状囊腺癌的腹水细胞为材料 ,进行体外培养 .将永生化基因———SV4 0T抗原基因转染第 2代细胞 ,得到永生化细胞系 .通过光学显微镜、生长曲线测定、染色体分析、双层软琼脂培养、裸鼠接种、免疫组化等 ,研究其生物学特性 ,并与其来源细胞的生物学特性进行比较 .建立了一株人卵巢浆液性囊腺癌永生化细胞系 ,命名为BUPH∶OVCA 3,现已传至 6 0余代 .其生物学特性为 ,细胞生长旺盛 ;具有人体恶性细胞的核型特征 ;细胞恶性度较低 ,不具有集落形成能力及裸鼠接种致瘤性 ;除较未永生化细胞生长速率增快 ,饱和密度增加外 ,仍保留上皮细胞的分化表型 .结果表明 ,BUPH∶OVCA 3为一株恶性度较低的人卵巢浆液性囊腺癌永生化细胞系 ,保留其来源细胞的生物学特性 。

To establish an immortalized human ovarian serous cystadenocarcinoma cell line, and study its biological characteristics, a specimen derived from ascites of a human ovarian serous papilliform cystadenocarcinoma was obtained and cultured in vitro . The 2nd passage was transfected with plasmid pcD2 SV40, colonies were expanded to immortalize cell lines. The morphology of the cells was observed by light microscope. Growth curve, karyotype analysis, culturing in soft agar, nude mice transplantation, immunohistochemistry were used to investigate its biologic characteristics. After transfection and selection, an immortalized ovarian serous cystadenocarcinoma cell line BUPH∶OVCA 3 was established. The cell line has been maintained for over 60 passages. The cells grew exuberantly and had malignant human karyotype characteristics. The cells could not form colony in soft agar and transplanted tumor was not observed in nude mice. Comparative studies revealed that BUPH∶OVCA 3 cells had an increased growth potential and higher saturation density, but the same differentiated phenotypes as its original cells. BUPH∶OVCA 3 was demonstrated as an immortalized human ovarian serous cystadenocarcinoma cell line with lower malignant. It retains similar properties of their original cells and could be a useful model for the study in human ovarian cystadenocarcinoma with lower malignant.