金雀异黄素对小鼠红白血病细胞系MEL的抑制作用

The Effect of Genistein on Mouse Erythroleukemia Cell Line

选择小鼠红白血病细胞系MEL作为金雀异黄素 (genistein)的作用对象 ,以探讨其对MEL的抑制作用及其可能的作用机理 .MTT和生长曲线表明 ,金雀异黄素对MEL细胞具有很强的生长抑制作用 (半数最大抑制效应浓度IC50 为 7 12mg L) .流式细胞术显示 ,金雀异黄素能够使MEL细胞发生G2 M期阻滞 ,但是诱导MEL凋亡作用并不明显 .DNA凝胶电泳发现金雀异黄素能够造成MEL细胞DNA的损伤 .联苯胺染色显示金雀异黄素对MEL细胞具有很强的诱导分化作用 ,且这种作用是时间和剂量依赖性的 .RT PCR显示 ,金雀异黄素能够抑制MEL细胞中原癌基因c myc的表达 .生物流变学检测发现 :金雀异黄素作用后 ,MEL细胞表面电荷密度减少、渗透脆性增大。

Mouse erythroleukemia cell line MEL was chosen as the target of genistein, to study its suppression effects, and the possible mechanisms. It was proved that genistein can greatly inhibit the growth and proliferation of MEL cell ( IC 50 is 7 12?mg/L) by use of MTT and growth curve. Flow cytometry analysis proved that Genistein can induce a G 2 M phase cell cycle arrest, but has little effect on apoptosis. Gel electrophoresis indicated that genistein can cause genomic DNA damage. Benzidine dying proved that genistein has a promising effect of inducing MEL to differentiate into erythoid cell in a time and dose dependent manner. RT PCR showed genistein can inhibit the c myc expression in MEL cell. The biorheology measurements indicated that cell surface charge density decreased, the osmotic fragility increased, and the cell maximum deformation decreased.