鸡贫血病毒vp3基因体内抑瘤效应的实验研究

Experimental Study on the Antitumor Effect of Chicken Anemia Virus vp3 Gene in vivo

目的 观察鸡贫血病毒 ( chicken anem ia virus,CAV) vp3基因的体内抑瘤效应。方法 分别将 pc DNA- vp3(含 vp3基因的真核表达载体 )、pc DNA3 (空载体 )和小鼠腹水型肝癌细胞株 H2 2混合后 ,接种于 BAL B/ c小鼠皮下。几天后 ,处死动物 ,取肿瘤组织称重 ,比较试验组和对照组瘤重差异 ,确定 vp3基因的抑瘤效应。TU NEL 法鉴定 vp3在体内诱导肿瘤细胞死亡的方式。结果 注射 vp3基因的试验组肿瘤生长率明显低于注射空载体的对照组。TUNEL 试验的结果也表明 ,鸡贫血病毒 VP3蛋白在体内以凋亡方式诱导肿瘤细胞死亡。结论 预示

Objective To observe the antitumor efficiency of chicken anemia virus vp3 gene in vivo. Methods Recombinant pcDNA-vp3 containing chicken anemia virus vp3 gene and control vector pcDNA3 was mixed with murine liver carcinoma cell lines H22 respectively, and the mixture was injected subcutaneously into Balb/c mice. After some days, the mice were sacrificed, and solid tumor weighed. The antitumor efficiency was calculated. At the same time, the way of tumor cell death induced by CAV VP3 in vivo was testified by TUNEL. Results The injection of pcDNA-vp3 and H22 could significantly reduce tumor growth in mice. TUNEL assay revealed that VP3 induced apoptosis in vivo. Conclusion CAV vp3 gene may be a new gene in inhibiting tumor cell growth in vivo.