电镜细胞化学及免疫标记检测在急性白血病诊断中的作用

The Role of Ultrastructural Cytochemistry and Immunological Method in the Diagnosis of Acute Leukemia

目的 探讨电镜细胞化学及免疫标记检测在急性白血病诊断中的意义。方法 对临床初治急性白血病( AL)在形态学分类的基础上结合有关免疫标记的检测开展了电镜髓过氧化物酶 ( MPO)、血小板过氧化物酶 ( PPO)细胞化学的研究。结果 发现有 2例细胞学分别诊断为 L1 及 L2 的病例被重新诊断为轻微分化型急性髓系白血病 ( M0 ) ,1例疑诊为急性原巨核细胞白血病的病例通过电镜 PPO阳性得到确诊。结论 白血病细胞免疫标记的检测及电镜细胞化学对急性白血病分类中髓系特征的判断具有敏感性好。

Objective To study the application of ultrastructural cytochemistry and immunological method in the diagnosis of acute leukemia. Methods Twenty-eight cases of clinically initiated treated acute leukemia diagnosed under routine light microscopy received further classification examination by using the immunological and ultrastructural methods. Results Two cases were classified as minimally differentiated acute myeloid leukemia (AML-M 0) previously diagnosed as L 1 and L 2 cytologically, one as megakaryoblastic leukemia which had failed to be discemed on simple morphological ground. The diagnosis of the remaining 25 cases were consistent with that based on light microscopy. Conclusion Immuno-labeled antibody and electron microscopy cytochemistry are sensitive and specific to the diagnosis of acute leukemia.