人脐静脉内皮细胞受剪切力作用后原癌基因c-fos和c-myc蛋白的表达

EXPRESSION OF PROTO-ONCOGENES C-FOS AND C-MYC IN CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS UNDER FLUID SHEAR FORCE

本文的研究目的是揭示不同的流动剪切力对体外培养的人脐静脉内皮细胞c fos和c myc蛋白表达的不同影响。我们利用平行板流动小室装置控制剪切力大小和持续时间。然后用免疫组织化学方法对受剪切力作用后的内皮细胞进行染色并进行图像分析。得到一些重要结果。c fos蛋白表达在静态时非常低 ,4dynes/cm2和 10dynes/cm2 的剪应力都可诱导c fos蛋白水平迅速增加 ,尤其是 10dynes/cm2 的剪应力。并随时间的增加而增加 ,到 1.0h达到高峰 ,随后下降 ,2 .5h时接近基础水平。c myc蛋白在静态时表达也非常低 ,经剪切力作用后缓慢增加 ,c myc蛋白水平明显较c fos低 ,而且剪应力为 4dynes/cm2 和 10dynes/cm2 两种情况对c myc蛋白表达的影响基本无差异。其高峰在 1.5h。随后下降 ,在 2 .5h时接近基础水平。结果表明人脐静脉内皮细胞c fos蛋白表达具有对剪切力大小和时间的依赖性 ;c myc蛋白表达具有时间依赖性。

The objective of this article is to reveal the different effects of different fluid shear forces on the expression of proto-oncogenes c-fos and c-myc in cultured human umbilical vein endothelial cells(HUVEC). The parallel plate flow chamber was used to control the value and duration of shear stress (SS). Then, c-fos protein and c-myc protein levels were measured by immunohistochemical methods and image analysis software. Some important conclusions were drawn. In the stationary state, c-fos protein levels were very low. The SS's of 4 dynes/cm2 and 10 dynes/cm2 induced rapid increases of c-fos protein levels, especially the SS of 10 dynes/cm2. The levels peaked at 1. 0h. Then, c-fos protein levels began to decrease, after 2. 5h, they declined to almost basal levels. In the stationary state, the c-myc protein levels were also very low; it slowly increased after the onset of shear stress. The effects of 4 dynes/cm2 and 10 dynes/cm2 SS on c-myc protein expression levels had no difference and apparently were less than that on c-fos protein levels. The c-myc protein levels peaked at 1. 5h. Subsequently, they fell to basal levels at 2. 5h. The results indicate that the expression of c-fos protein in cultured HUVEC is dependent on the value and duration of shear stress, while c-myc is dependent on the duration of shear stress.