Ⅳ型胶原酶和E-CD在膀胱移行细胞癌侵袭转移中的作用

The Expression of MMP2, MMP9, Type Ⅳ Collagen and E - CD in Bladder Transitional Cell Carcinoma and Its Clinical Significance

目的 探讨Ⅳ型胶原酶(MMP2,MMP9)、E-CD在膀胱移行细胞癌(BTCC)侵袭转移中的作用及在临床应用中的价值。方法采用免疫组化方法对49例膀胱移行细胞癌及10例正常膀胱粘膜组织中MMP2,MMP9、Ⅳ型胶原及E-CD进行染色。观察(1)MMP2,MMP9、Ⅳ型胶原和E-CD同肿瘤分级分期的关系。(2)MMP2,MMP9同Ⅳ型胶原的表达之间的关系。(3)MMP2,MMP9、Ⅳ型胶原和E-CD的表达与患者术后复发及预后之间的关系。结果 随肿瘤分级的增高MMP9和MMP2的表达显著增强(P<0.01和P<0.05)。随肿瘤分期的增高MMP2的表达显著增强(P<0.05)。随肿瘤分级、分期的增加Ⅳ型胶原的表达显著减少(P<0.01和P<0.01)。E-CD的表达亦显著减弱(P<0.01和P<0.05)。MMP2的高表达同Ⅳ型胶原染色的减少显著相关(r=O.466 P<0.01)。MMP2高表达是患者预后不良的危险因素(P<0.05)。结论 MMP2在BTCC的侵袭过程中起到了重要的作用,MMP2可能成为估计BTCC预后的一项有用的指标。

Objective To explore the clinical value of the expression of Matrix metalloproteinase 2,9, type Ⅳ collagen and E - CD in the bladder transitional cell carcinoma. Methods MMP2, MMP9, type Ⅳ collagen and E - CD were examined immunohistochemically in 49 cases of BTCC and 10 cases of normal bladder tissue.The correlation between the expression of MMP2,MMP9,type Ⅳ collagen,E - Cd and tumor grade and stage, the correlation between MMP2, MMP9 and type Ⅳ collagen, the correlation between MMP2, MMP9, type Ⅳ collagen, E - CD and tumor recurrence and patients' survival rate were closely studied.Results The expression of MMP2,MMP9 increased with tumor grade ( P < 0.01 and P < 0.05) ,MMP2 also increased with stages ( P < 0.05 ) . The expression of type Ⅳ collagen reduced with tumor grade and stage (P < 0.01 and P < 0.01) . Reduction of type Ⅳ collagen correlated significantly with increased expression of MMP2 ( r = 0.466 P < 0.01). High expression of MMP2 was associated with poor outcome ( P < 0.05) .Conclusions MMP2 played a key role in the invasion of BTCC.MMP2 may be a useful predictor in bladder transitional cell carcinoma.