蛋白质组研究中的二维电泳分离技术

Two-Dimensional Electrophoresis Separation Technique in Proteome Research

对目前生命科学研究中的热点领域蛋白质组研究中应用的主要分离技术二维聚丙烯酰胺凝胶电泳的基本原理、应用和最新进展进行了综述。同时,针对二维凝胶电泳的缺陷,介绍了目前正在发展的几种替代技术。

As the major separation method in the technique platform of proteome, twodimensional polyacrylamide gel electrophoresis (2DE) was optimized and developed in many ways in recent years. Basic principles, applications and new advances of 2DE are reviewed. In regard to the weakness of 2DE, some alternative separation methods are also introduced.