涂敷型淀粉类液相色谱手性固定相对丙烯菊醇对映体的拆分

Chiral Separation of 2-Allyl-4-Hydroxy-3-Methyl-2-Cyclopenten-1-one on Coated Amylpectin Chiral Stationary Phase in High Performance Liquid Chromatography

合成了直链淀粉 三(3,5 二甲基苯基氨基甲酸酯),涂于氨基丙烷化硅胶上制得高效液相色谱手性固定相,并利用该固定相对外消旋丙烯菊醇对映体进行了拆分。考察了流动相中异丙醇含量对分离效果的影响。

Allethrin is an important insecticide and its two enantiomers have different bioactivities. The allyl4hydroxy3methyl2cyclopenten1one is an important intermediate in chiral synthesis of allethrin, and its optical purity is closely related to that of the final product. So it is very important to establish a method to separate the racemates of 2allyl4hydroxy3methyl2cyclopenten1one. Amylpectin tris (3,5dimethylphenylcarbamate) was synthesized and coated on spherical silica gel as a chiral stationary phase (CSP),with which a practical and useful chiral column was packed for high enantiomeric resolution. The effects of isopropanol concentration in the mobile phase on the retention and resolution were investigated. The retention factors of the racemates decreased appreciably with the increment of the concentration of isopropanol and so was the resolution factor.