甲基叔丁基醚无铅汽油肾脏毒性的实验研究

Experimental study on kidney toxicity of methyl tertiary-butyl ether (MTBE) lead free gasoline

目的 了解甲基叔丁基醚(MTBE)无铅汽油对肾脏的毒性及毒作用机制。方法 昆明种小鼠经呼吸道静式染毒,MTBE无铅汽油22.9、11.4及2.3g/m3每天一次,连续2h,共22 d亚急性染毒。日立-7150型全自动生化仪检测血清中尿素氮(BUN)、肌酐(Cr)含量;肾组织匀浆中丙二醛(MDA)和超氧化物歧化酶(SOD)含量分别用荧光法、邻苯三酚自氧化固定时间法测定;电镜观察肾皮质区超微结构的变化。结果22.9g/m3染毒组雌性小鼠血清中BUN含量与阴性对照组间比较差异有显著性(P<0.05);电镜观察到22.9g/m3染毒组与对照组雌雄性小鼠的肾小球基底膜、肾小管细胞线粒体及绒毛均未见显著异常改变。结论 MTBE无铅汽油对肾小球的滤过功能有一定的影响,雌性小鼠可能更为敏感。

Objective To study the kidney toxicity and toxic mechanism induced by methyl tertiary-butyl ether (MTBE) lead free gasoline.Methods Subacute intoxication by static inhalation of MTBE lead free gasoline on Kunming mice, dosing 22.9, 11.4 and 2.3 g/m3 , 2 hours a day, for 22 days. The blood urea nitrogen (BUN) and critinine (Cr) in serum were detected, the contents of su-peroxide dismutase (SOD) and malondialdehyde (MDA) in homogenate of kidney were determined by the pyrogallol autoxidation method at fixed time and fluorometry respectively, meanwhile the damage of ultrastructure of nephric cortex cells was observed with electron microscopy . Results The BUN in serum of the female mice exposed to 22.9 g/m3 MTBE lead free gasoline was lower than that of control group (P < 0.05). No obvious change was observed in ultrastructure of nephric cortex cells. Conclusion MTBE lead free gasoline might affect the glomerular filtrating function, and the female mice appeared to be more sensitive than the male mice.