甲氨碟呤脂质体的几种制备方法比较

The comparison of some methods about liposome preparation

目的探讨脂质体的制备方法 ,以便在具体工作中根据不同的目的选择适当的制备方法。方法采用逆相蒸发法、注射法、薄膜法和超声法制备包裹了甲氨喋呤和环胞苷的脂质体。对各种方法的关键步骤进行了讨论。结果被包物质如不稳定 ,采用薄膜法轻轻振摇超过 10h ,包封率可比短时间或震荡激烈提高。被包物质如经过短时间的超声和有机溶剂处理 ,采用逆相蒸发法 ,可获得较高包封率。结论根据被包物质的性质及具体工作不同 ,选择适当的脂质体制备方法 。

PurposeThe preparation methods of liposome were explored in order to select suitable technique based on different objective under working conditions.MethodsThe liposomes of MTX and Ara c were wrapped up by adopting reverse phase evaporation, injection, film dispersion and ultrasonic dispersion. and the key steps about the preparation methods were discussed.ResultsEncapsulation efficiency was raised by film dispersion of vibrating gently to exceed 10 hours on condition that the wrapt compounds were unstable, compared with the methods of short time vibrate or drastic vibrate. The high encapsulation efficiency was obtained by reverse phase evaporation if the wrapt compounds were stable under ultrasonic dispersion of short time and organic solvent.ConclusionThe preparation methods of liposome should be determined by the properties of wrapt compounds and working conditions in order to achieve higher encapsulation efficiency and working efficiency.