人工培养蛹虫草多糖的分离纯化及其结构的初步研究

Separation, purification and structural analysis of polysaccharides in cultured Cordyceps militaris L.Link

目的人工培养蛹虫草多糖类成分的提取与纯化。方法采用SephadexG 10 0柱色谱法进行了分离纯化 ;通过理化常数测定、化学和光谱分析等方法研究了其化学结构特征。结果分离得到CPS 1、CPS 2和CPS 33种多糖 ,其单糖组成分别为 :CPS 1:鼠李糖 木糖 甘露糖 葡萄糖 半乳糖 (1∶6 .4 3∶2 5 .6∶16 .0∶13.8) ;CPS 2 :鼠李糖 葡萄糖 半乳糖 (1∶4 .4 6∶2 .4 3) ;CPS 3为只含葡萄糖的均一多糖。 3种多糖的分子量分别为 2 30 0 0、12 90 0和 5 0 0 0。CPS 1主要含有β糖苷键 ,CPS 2和CPS 3主要含有α糖苷键。 结论CPS 1为一种含有 5种单糖 ,糖苷键为 β构型的多糖 ;CPS 2是一种含有 3种单糖 ,糖苷键为α构型的多糖 ;CPS 3是一种仅含葡萄糖。

PurposeTo conduct separation, purification and structural analysis of polysaccharide constituents in cultured Cordyceps militares L. Link. MethodsIndividual constituents were isolated and purified by gel filtration chromatography, and its structural features were further studied by physicochemical constants, and spectral analysis.ResultsThree polysaccharides, named CPS 1,CPS 1 and CPS 3 were obtained from boiling water extract. All structures were found to be complex neutral heteropolysaccharides. CPS 1: Rha Glc Xyl Man Gal(1∶6.43∶25.6∶16.0∶13.8). CPS 2: Rha Glc Gal(1∶4.46∶2.43). And CPS 3 were composed by Glc only. IR indicated that CPS 1 had βconfiguration and CPS 2 and CPS 3 had αconfiguration.ConclusionCPS 1 was a polysaccharide consisting of five monosacchides connected by β configuration; CPS 2 was a polysaccharide composed of three monosaccharedes connected by α configuration; CPS 3 was a polysaccharide composed only of glucose connected by α configuration.