摘要
为探讨慢性乙型肝炎活检组织HBcAg定量检测与细胞凋亡率及凋亡因子Apo2 7表达的相关性。文章将 37例慢性乙型肝炎患者肝活检组织制备成单细胞悬液 ,用小鼠抗人HBcAg单克隆抗体标记后 ,用AnnexinV FITC/PI双染法分辨凋亡细胞及坏死细胞 ,并以凋亡因子Apo2 7标记上述细胞 ,均应用流式细胞术进行了定性定量检测。结果是慢性乙型肝炎患者肝活检组织细胞中 ,存在着不同程度和比例的坏死细胞和凋亡细胞 ,重度慢性乙型肝炎坏死细胞和凋亡细胞比例显著多于轻度慢性乙型肝炎者 (P <0 0 1)。慢性乙型肝炎活检组织HBcAg含量与肝细胞凋亡率、Apo2 7因子表达呈显著相关性 ,Apo2 7表达水平随病损程度的加重而增加 ,有统计学差异。文章提示慢性乙型肝炎活检组织HBcAg含量与肝细胞凋亡率、Apo2 7因子表达呈显著相关性 ,有助于判断病损程度和观察疗效 。
To investigate the correlation between the HBcAg content and its cellular apoptotic rate (CAR),as well as the expression of Apo2 7 factor in liver needling biopsy tissue with chronic hepatitis B (CHB),thirty seven patients with CHB were performed liver needling biopsy and the tissues were made into cellular suspension Then immunologically marked by fluorescent monoantibody of HBcAg and Apo2 7 (a mitochondral membrane protein),as well as painted by AnnexinV FITC/Propidium iodide(PI) and assayed its CAR,which could distinguished early apoptotic cells from necrotic cells All were quantitatively measured by flow cytometry(FCM) At the same time the common HE sections were pathologically examined The results were that:there was different percentage of apoptotic cells from necrotic cells in liver needling biopsy tissue with CHB The percent of necrotic cells and CAR in III degree damage patients were statistically more than that in I degree damage ones ( P <0 01) The HBcAg content was statistically correlated with its CAR and the expressive level of Apo2 7 factor This article suggested that the analysis of CAR and the expressive level of Apo2 7 factor could directly determine the tissue damage degree and detected curate effect,which might has important clinic significance
出处
《上海免疫学杂志》
CSCD
北大核心
2003年第1期47-49,共3页
Shanghai Journal of Immunology
