慢性肾衰患者外周血IL-18水平及血液透析对其的影响

Study on the Effect of Hemodialysis to the Peripheral Blood Level IL-18 in Patients with Chronic Renal Failure

为探讨慢性肾衰竭 (CRF )患者外周血IL 18表达量的变化以及血液透析 (HD )对其表达的影响 ,选取 10名健康志愿者及 2 9例CRF患者 ,应用ELISA测定血浆IL 18水平 ,同时采用半定量逆转录多聚酶链反应 (RT PCR )技术 ,检测PBMC中IL 18mRNA表达量。结果是未行HD的CRF患者血浆IL 18水平及PBMCIL 18mRNA表达量较正常对照组增高 ,差异有显著统计学意义 (P <0 0 1) ,单次HD对CRF患者血浆IL 18水平及基因表达无明显影响 (P >0 0 5 ) ,但长期维持HD则可使CRF患者外周血IL 18水平及基因表达增高 (P <0 0 5 )。提示外周血IL

To investigate peripheral blood gene expression of interleukin 18 (IL 18) and the effect of hemodialysis (HD) to its expression in patients with chronic renal failure (CRF),the plasma level of IL 18 and expression of IL 18 mRNA in peripheral blood mononuclear cells (PBMC) were evaluated by enzyme linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT PCR) respectively in 10 healthy volunteers and 29 patients with chronic renal failure The results showed that the plasma level of IL 18 and expression of IL 18 mRNA in PBMC in patients with chronic renal failure were higher significantly than those of healthy controls They were not obviously changed after the first HD,but further increased significantly after two months of hemodialysis It suggested that high level of IL 18 expression in peripheral blood may play a significant role in pathogenesis of CRF and dialysis related morbidity