铅暴露对学龄前两性儿童免疫系统的影响

EFFECT OF LEAD EXPOSURE ON IMMUNE SYSTEM OF PRESCHOOL CHILDREN.

目的 :探讨环境铅暴露对学龄前儿童免疫系统影响的性别差异。方法 :石墨炉原子吸收光谱仪对 2 17名学龄前儿童进行血铅测定 ,流式细胞仪检测铅中毒对不同性别学龄前儿童 T淋巴细胞亚群 CD+3、CD+3CD+4 、CD+3CD+8以及 B细胞 (CD- 3CD+1 9)、NK细胞 (CD- 3CD+1 6 CD+56 )的影响 ;散射比浊法检测免疫球蛋白 Ig G、Ig M水平。结果 :铅中毒的女童 CD+3CD+4 、CD+3CD+4 / CD+3CD+8、Ig G、Ig M与对照组比较均出现明显下降 (P<0 .0 5 ) ,而男童与对照组间差异无显著性 (P>0 .0 5 )。结论

Objective:To study the effect of lead exposure on immune system in preschool children of different sex.Methods:Blood lead level was measured in 217 preschool children by graphite furnace atomic absorption spectrophotometry.Tlymphocyte subsets CD + 3?CD + 3CD + 4?CD + 3CD + 8,B cells(CD - 3CD + 19 ),NK cells(CD - CD + 16 CD + 56 ) in children with lead poisoning were analyzed by flow cytometer.Serum immunoglobulins IgG?IgM levels were determined by turbidimetry.Results:CD + 3CD + 4?CD + 3CD + 4/CD + 3CD + 8?IgG?IgM were significantly lower in female of lead poisoning group compared to control group( P <0 05),but no differences were found in male( P >0 05).Conclusions:Lead poisoning can cause unfavorable effect on immune system and the effect of lead in girls was stronger than that in boys.