鼻息肉组织炎性细胞中血管通透性因子的表达

Expression and significance of vascular permeability factor (VPF) in inflammatory cells of nasal polyps

目的 :探讨鼻息肉组织嗜酸性粒细胞、巨噬细胞和浆细胞中血管通透性因子 (VPF)的表达及意义。方法 :12例鼻息肉标本行VPF的免疫组化染色、双重免疫酶染色以及免疫组化和MGG(may gr櫣ｎｗａｌｄｇｉｅｍｓａ)双重染色 ,光镜观察。结果 :VPF在鼻息肉组织嗜酸性粒细胞、巨噬细胞及浆细胞中均有表达。结论 :VPF可能是嗜酸性粒细胞、巨噬细胞及浆细胞发挥促息肉形成作用的途径之一。

Objective:To study the expression and significance of vascular permeability factor (VPF) in eosinophil, macrophage and plasma cell in nasal polyps.Method:VPF in 12 nasal polyps was studied by immunohistochemistry, double immune enzymatic staining technique and double MGG staining technique. Each section was observed under optical microscope.Result:Immunohistochemistry result: VPF was detected in the cells showing morphological feature of plasma cells. Double immune enzymatic staining result: CD 68 positive cells in the second staining conformed to VPF positive cells in the first staining. MGG and immune enzymatic double staining result: eosinophils in MGG staining were in conformity with some VPF positive cells.Conclusion:Positive expression of VPF in eosinophils, macrophages and plasma cells indicates that VPF may play a role in the formation of heavy edema of nasal polyps.