高效液相色谱法测定狗体内大黄游离蒽醌的含量

Determination of Anthraquinone Free Derivatives From Chinese Herbal in Dog Serum by HPLC

目的 :建立狗体内大黄游离蒽醌的含量测定方法。方法 :采用HypersilBDSC18 色谱柱 (4 6mm×250mm) ,甲醇 -0 5 %冰醋酸 (80∶20)为流动相 ,流速为1 0ml/min ,检测波长为430nm。样品以甲醇沉淀蛋白并提取 ,常温下氮气吹干 ,复溶进样分析。结果 :芦荟大黄素在0 15～6 0μg/ml(r=0 9996)、大黄酸在0 14～5 6μg/ml(r=0 9982)、大黄素在0 13～5 2μg/ml(r=0 9990)、大黄酚在0 2～8 0μg/ml(r=0 9991)、大黄素甲醚在0 1～4 0μg/ml(r=0 9994)范围内线性关系良好。结论 :该方法为大黄制剂中游离蒽醌成分的体内分析及其生物利用度研究提供了方法依据。

OBJECTIVE:To establish a HPLC method for determination of anthraquinone free derivatives from Chinese herbal in dog serum METHODS:With Hypersil BDS C18 column,the mobile phase was methanol-0 5% acetic acid(80∶20) The sample was extracted with methanol and dried at room temperature The residue was reconstituted by methanol and analyzed at 430nm RESULTS:The five components can be separated well The linear ranges were Aloe-emodin(0 15～6 0μg/ml,r=0 9 996) Rhein(0 14～5 6μg/ml,r=0 9 982);Emodin(0 13～5 2μg/ml,r=0 9 990);Chrysophanol(0 2～8 0μg/ml,r=0 9 991);Physcion(0 1～4 0μg/ml,r=0 9 994) CONCLUSION:This method can provide the basis for vivo analysis of anthraquinone free derivatives and bioavailability study of Rheum palmatum preparations