虎杖的化学成分研究

Studies on the chemical constituents of Polygonum cuspidatum

目的 研究蓼科植物虎杖的化学成分。方法 利用反相色谱的方法进行分离纯化 ,根据化合物的化学性质与光谱数据鉴定其结构 ,DNA裂解活性采用改进的Hecht法。结果 自虎杖根茎的 60 %丙酮提取物中分得 1 0个化合物 ,它们结构分别为没食子酸 (1 ) ,色氨酸 (2 ) ,2 ,6 二羟基苯甲酸 (3) ,(+) 儿茶素 (4) ,大黄素 (5) ,大黄素 8 O β D 吡喃葡萄糖苷 (6) ,(+) 儿茶素 5 O β D 吡喃葡萄糖苷 (7) ,1 (3 O β D 吡喃葡萄糖基 4,5 二羟基 苯基 ) 乙酮 (8) ,tachioside(9)和isotachioside(1 0 )。 结论 化合物 1 ,2 ,3 ,8,9,1 0为首次从该植物中分得 ,化合物 1 ,4。

OBJECTIVE: To study the chemical constituents of Polygonum cuspidatum. METHOD: The constituents were separated by means of column chromatography on several reverse phase gels, identified by the analysis of spectral data and compared with literature data. The DNA cleavage activity was evaluated through a modified Hecht method. RESULTS: Ten compounds were isolated and their structures were elucidated as gallic acid(1), tryptophan(2), 2,6-dihydroxy-bezoic acid(3), (+)-catechin(4), emodin(5), emodin-8-O-β-D-glucopyranoside (6), (+)-catechin-5-O-β-D-glucopyranoside (7), 1-(3-O-β-D-glucopyranosyl-4, 5-dihydroxyphenyl)-ethanone(8), tachioside(9) and isotachioside(10) respectively. CONCLUSION: Compounds 1,2,3,8,9 and 10 were isolated from the plant for the first time. Compounds 1,4 and 7 showed significant DNA cleavage activity.