葛根素注射液对慢性乙醇中毒小鼠记忆障碍的改善作用

Investigation on effects of puerarin against memory impairment in mice induced by chronic alcoholism

目的 探讨葛根素注射液对慢性乙醇中毒小鼠记忆障碍的改善作用及其脑内机制。方法 每日给予 30 %乙醇连续 4周造成小鼠慢性乙醇中毒 ,同时每日ip 2 0 ,1 0 0mg·kg- 1 葛根素注射液治疗 4周 ,用Y 迷宫检测小鼠分辨学习和记忆行为的变化 ,用紫外分光光度计和荧光分光光度计分别检测脑组织SOD ,脂褐素生化指标 ;用电镜和计算机图像分析仪观测小鼠海马CA3区GrayⅠ型突触界面结构参数的变化。结果 葛根素注射液 1 0 0mg·kg- 1 可显著改善长期给予乙醇造成的小鼠学习记忆功能障碍 ;显著提高脑组织SOD的活性 ,使小鼠脑内脂褐素含量明显下降 ;同时使海马突触后致密物质 (PSD)厚度显著增加 ,突触间隙宽度显著减小。结论 葛根素注射液能改善慢性乙醇中毒引起的小鼠学习记忆功能障碍 ,该作用可能与其提高脑组织抗氧化能力 。

OBJECTIVE: To study the improvement effects of puerarin (Pue) on impairment of learning-memory in mice induced by long-term administration of alcohol, and its possible mechanism of action. METHOD: Chronic alcoholism in mice were induced by long-term i.g. administration of alcohol for 4 weeks. Meanwhile, the mice were treated with Pue 20 or 100 mg&middotkg-1 once daily for 4 weeks. After the last treatment, learning-memory behavior was tested using Y-maze. Then the activity of SOD and the content of lipofuscin were measured by spectrophoto-metry and Fluorospectrophotometer, respectively. And the synaptic interlace structure of Gray I in CA3 area of hippocampus was quantitatively analyzed by electronic microscope and computer image processing appliance. RESULTS: Long-term administration of alcohol caused impairment of learning-memory in mice, declined the activity of SOD, and increased the level of lipofuscin. It was found that Pue 100 mg&middotkg-1 improved remarkably learning-memory ability of the mice, promoted the activity of SOD, decreased the content of lipofuscin, increased significantly the thickness of post-synaptic density (PSD), and shorten the width of the synaptic cleft in hippocampus CA3 area. CONCLUSION: Pue showed an improvement effect against the memory impairment in mice induced by long-term administration of alcohol. Improvement of the antioxidase activity in brain and an alteration of synaptic interface structure of hippocampus may be related.