期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

溶血磷脂酸对豚鼠心室肌细胞钙电流的影响 被引量：2

Effect of Lysophosphatidic Acid on Calcium Current in Isolated Ventricular Cells of Guinea Pig
下载PDF
导出
摘要 目的 :研究溶血磷脂酸 ( LPA)对心肌细胞膜离子通道的作用及机制。方法 :采用全细胞膜片钳技术。结果 :0 .1、1 .0和 1 0 .0 μmol·L-1的 LPA对豚鼠心室肌细胞 Ca2 +通道电流均具有明显的增强作用 ,而且这种作用可以被 G蛋白受体阻断剂白喉毒素阻断。结论 :LPA通过与 G蛋白偶联的膜受体介导促进豚鼠心室肌细胞 Ca2 + Objective: To study the effect of lysophosphatidic acid and their mechanisms. Methods:The whole cell voltage clamp technique was used in isolated ventricular cells of guinea pig. Results:LPA ( 0.1 ,1.0 and 10.0 μmol·L -1 ) showed significant enhancement to the calcium current in dose dependece manner, and the effect was blocked by PTX, a Gprotein coupled receptor blocking agent. Conclusion:LPA can promote the opening of calcium channels of the ventricular cells by G protein coupled membrane receptor.
作者 李英骥 陈霞 葛敬岩 闫励 张文杰 钟国赣
机构地区 吉林大学基础医学院生理学教研室
出处 《吉林大学学报（医学版）》 CAS CSCD 北大核心 2003年第1期62-64,共3页 Journal of Jilin University：Medicine Edition
关键词 溶血磷脂酸 膜片钳术 心室肌细胞 溶磷脂素类 钙通道 lysophosphatidic acid, patch clamp techniques ventricular cells lysophospholipids calcium channels
分类号 Q463 [生物学—生理学] R－332 [医药卫生]
  • 相关文献

参考文献7

  • 1[1]Eichholtz T, Jalink K, Fahrenfort I, et al.The bioactive phospholipid lysophosphatidic acid is released from activated platelets[J]. B iochem J, 1993, 291:677-680.
  • 2[2]Edward J, Goetzl, Songzhu An. Diversity of cellular receptors and functions for the lysophospholipid growth factors lysophosphatidic acid and sphingosine 1-phosphate[J]. Faseb J, 1998,12:1589-1598.
  • 3[3]Moolen aar WH. Development of our current understanding of bioactive lysophospholipids [J]. Ann N Y Acad Sci,2000,905(4):1-10.
  • 4[4]Fedida D, Noble D, Shimoni Y, et al.Inward current related to contraction in guinea-pig ventricular myocytes[J]. J Physiol, 1987,385(4):565 -589.
  • 5[5]Lu Yang, Dina Andrews, Philip S. Low Lysophosphatidic acid opens a Ca2+ channel in human erythrocytes[J]. Blood, 2000,95(4):2420-24 25.
  • 6[6]Chiyoko N, Inoue HG, Forster ME. Effects of lysophosphatidic acid, a novel lipid mediator, on cytosolic Ca2+ and contractility in cultured rat mesangial cells[J]. Circulation Research, 1995,77:888.
  • 7[7]Yoh TN, Naotoshi S. The edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities1[J ]. J Biochem, 2002,131(6):767-771.

同被引文献16

  • 1Orrenius S, Nicotera P. The calcium ion and cell death [J].J NeuralTransmSuppl, 1994, 43: 1-11.
  • 2Cabo C, Wit AL. Cellular electrophysiologic mechanisms of cardiac arrhythmias[J]. Cardiol Clin, 1997, 15 (4): 517-538.
  • 3Spiegel S, Milstien A. Sphingosine 1-phosphate, a key cell signaling molecule [J]. J Biochem, 2002, 277 (29): 25851-25854.
  • 4Sanchez T, Hla T. Structural and functional characteristics of SIP receptors [J]. J Cell Biochem, 2004, 92: 913-922.
  • 5Hla T. Physiological and pathological actions of sphingosine 1-phosphate [J]. Semin Cell Dev Biol, 2004, 15 (5): 513-520.
  • 6Mattie M, Brooker G, Spiegel S. Sphingosine-l-phosphate, a putative second messenger, mobilizes calcium from internal stores via an inositol trisphosphate-independent pathway [J].J Biol Chem, 1994, 269: 3181-3188.
  • 7Piacentino V, Dipla K, Gaughan JP, et al. Voltage-dependent Ca2+ release from the SR of feline ventricular myocytes is explained by Ca2+-induced Ca2+ release[J]. J Physiol, 2000,523: 533-548.
  • 8atomi Y, Ozaki Y, Ohmori T, et al. Sphingosine 1-phosphate: synthesis and release [J]. Prostaglandins, 2001,64(1-4): 107-122.
  • 9Yatomi Y, Igarashi Y, Yang L, et al. Sphingosine-1-phosphate, a bioactive sphingolipid abundantly stored in platelets, is a normal constituent of human plasma and serum[J]. J Biochem, 1997, 121: 969-973.
  • 10Moolenaar WH. Lysophosphatidic acid, a multifunctional phospholipid messenger [J]. J Biol Chem, 1995, 270:12949-12952.

引证文献2

二级引证文献8

吉林大学学报（医学版）
2003年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部