十六烷基壳聚糖纳米微球负载紫杉醇的研究

Studies on cetyl-chitosan nanosphere as carriers for paclitaxel

目的 研究适用于水体系的紫杉醇的负载载体。方法 壳聚糖经烷基改性制得烷基取代的壳聚糖衍生物 ,该衍生物在水中可自动形成粒径分布在 10 0 nm左右的纳米微球 ,并在磷酸缓冲液 (p H=7.4 )中对负载紫杉醇的纳米微球进行了体外释放研究。结果 随着烷基取代度的增加 ,紫杉醇的体外释放平衡浓度降低 ,体外释放的半释放时间延长。结论 十六烷基壳聚糖纳米微球是一个优良的紫杉醇的水分散体系。

Object To prepare and study a suitable carrier for paclitaxel in water. Methods Cetyl chitosan (CTCS), prepared by reacting chitosan (CS) with chlorocetane under alkaline condition, was soluble and spontaneously formed nanosphere about 100 nm in diameter. And the release in vitro from paclitaxel loaded CTCS nanosphere was measured in phosphate buffer solution (PBS, pH=7 4). Results The balanced release concentration of paclitaxel was deceased and half release time (t 1/2 ) was delayed with the increase of substitution degree of alkyl. Conclusion This kind of nanosphere is an excellent carrier for paclitaxel in water.