液相色谱法制备丹参素钠

Preparative separation of D(+)-β-(3,4-dihydrophenyl)-lactate sodium from Salvia miltiorrhiza by HPLC

目的 分离纯化丹参中的水溶性活性成分丹参素钠。方法 采用亲脂性吸附树脂与制备型反相高效液相色谱组合分离技术 ,将丹参水提粗制品经亲脂性吸附树脂柱色谱预分离后 ,再由制备型 RP- HPL C纯化精制。结果产品经熔点、MS,IR,UV和 HPL C图谱鉴定 ,并与文献、标准对照品比较 ,证明为丹参素钠 ,纯度 >98%。结论 该方法简单 ,重复性较好 ,采用的溶剂系统价廉易得、沸点低、易于回收。

Object To develop a method for the isolation and purification of D(+) β (3,4 dihydrophenyl) lactate sodium from Salvia miltrorrhiza Bge. Methods Combined lipophilic adsorption resin and preparative RP HPLC, the water extract of S. miltrorrhiza was pre isolated by lipophilic adsorption resin, the enriched extract was purified to give D(+) β (3,4 dihydrophenyl) lactate sodium by preparative RP HPLC. Results By contrast with the literature and standard reference substance, D(+) β (3,4 dihydrophenyl) lactate sodium was identified by MS, IR, UV, HPLC and melting point. The final purity of the compound is >98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap, with low boiling point and easy to recovery property.