脓毒症性肺损伤大鼠炎性细胞因子的变化及己酮可可碱的干预作用

EFFECT OF PENTOXIFYLLINE ON THE EXPRESSIONS OF SEVERAL INFLAMMATORY CYTOKINE GENES IN RATS WITH SEPSIS-INDUCED ACUTE LUNG INJUR.

观察脓毒症性急性肺损伤 (ALI)大鼠肺组织及外周血炎性细胞因子的变化 ,探讨已酮可可碱 (PTX)的干预作用。采用盲肠结扎、穿孔的方法制成脓毒症性肺损伤模型 ,用ELISA法或胸腺细胞增殖法测定血清TNF α、IL 6、IL 8含量及IL 1活性 ;斑点杂交检测肺组织中TNF α、IL 1β、IL 6、IL 8mRNA表达。结果显示 ,①脓毒症各组肺组织内TNF α、IL 1β、IL 6、IL 8mRNA表达强度均显著高于对应时相点的假手术组 (P <0 0 1)。PTX干预后 ,肺组织内TNF α、IL 1β、IL 6、IL 8mRNA的相对含量较未用PTX干预的大鼠有一定程度的减少。②脓毒症各组血清中TNF α、IL 1、IL 6、IL 8含量或活性均显著高于对应时相点的假手术组 (P <0 0 1)。PTX 2 4h组血清中TNF α、IL 1、IL 6、IL 8含量或活性明显低于脓毒症 2 4h组 (P <0 0 1)。提示脓毒症性ALI大鼠肺组织内TNF α、IL 1β、IL 6、IL 8mRNA表达及血清相应细胞因子含量或活性均升高 ,PTX可在一定程度上抑制体内TNF α、IL 1、IL 6、IL 8的产生。

To investigate the expression of TNF α, IL 1β, IL 6 and IL 8 mRNA in the lung,and the levels of TNF α, IL 1, IL 6 and IL 8 in serum of rats with sepsis induced acute lung injury(ALI), and to examine the effect of pentoxifylline on the production of these cytokines. The expressions of TNF α, IL 1β, IL 6 and IL 8 mRNA were detected with dot blot.The levels of TNF α, IL 1β, IL 6 and IL 8 were examined with ELISA. Compared to the sham operation group, TNF α, IL 1β, IL 6 and IL 8 mRNA expression in sepsis group were increased evidently( P< 0 01), along with increase in these cytokines in the serum ( P< 0 01). PTX markedly inhibited TNF α, IL 1β, IL 6 and IL 8 mRNA expressions. The levels of these cytokines in serum were lower in PTX group than those in sepsis group ( P< 0 01). It is the results demonstrated that TNF α, IL 1β, IL 6 and IL 8 mRNA expressions were increased in the lung in rats with sepsis induced ALI, and the levels of these cytokines in serum were increased sustainedly. PTX might inhibit the production of these inflammatory cytokines to certain extent.