LPS、TNF-α、IL1-β对大鼠多核白细胞环氧合酶2表达的影响

EFFECTS OF LPS, TNF-α, IL-1β ON CYCLOOXYGENASE-2 EXPRESSION IN RAT POLYMORPHONUCLEAR LEUKOCYTES

为探讨LPS、TNF α、IL 1β对多核白细胞 (PMN)环氧合酶 2 (COX 2 )表达及前列腺素 (PGs)的影响 ,以LPS、TNF α、IL 1β刺激大鼠PMN 2 4h后 ,采用RT PCR检测PMNCOX 2mRNA表达情况并观察PGs的变化。结果发现 ,静息状态下PMN仅表达极少量的COX 2mRNA ;受炎性刺激后 ,PMN大量表达COX 2mRNA ,同时伴有PGs的升高。说明炎性刺激可诱发PMN的COX 2高表达和PGs升高 ,提示COX

To explore the effects of lipopolysaccharide (LPS), TNF α and IL 1β on cyclooxygenase 2 (COX 2) expression and prostaglandins (PGs) in rat polymorphonuclear (PMN), measured COX 2 mRNA was determined by reverse transcription polymerase chain reaction and changes in PGs after stimulation of PMN with LPS, TNF α and IL 1β for 24 hours were observed. The resting PMN were found to express COX 2 mRNA weakly, while it was significantly up regulated after the stimulation of LPS,TNF αand IL 1β. The level of PGs was increased markedly at the same time. These results suggested that COX 2 might play an important role during inflammatory process involving PMN.