脂多糖对复合培养的肺微血管内皮细胞和肺动脉平滑肌细胞生长周期的影响

EFFECTS OF LIPOPOLYSACCHARIDE ON LUNG MICROVASCULAR ENDOTHELIAL CELLS AND PULMONARY ARTERIAL SMOOTH MUSCLE CELLS BY COCULTURE

以脂多糖 (LPS)处理复合培养的大鼠肺微血管内皮细胞 (LMVEC)和肺动脉平滑肌细胞 (PASMC) ,利用流式细胞仪检测LMVEC和PASMC细胞周期的变化。结果发现 ,单独培养和复合培养时 ,LPS均能使G1期细胞减少 ,S +G2 /M期细胞增多 ;LPS处理 16h后 ,复合培养组LMVEC和PASMC较单独培养组G1期细胞增多 ,S +G2 /M细胞数减少 ;LPS可使单独培养的LMVECS期细胞增多。说明LPS可促进LMVEC和PASMC分裂、增殖 ,二者复合培养可减轻LPS引起的LMVEC和PASMC增殖作用 ;LMVEC和PASMC在细胞增殖方面存在着复杂的调节关系。

Rat lung microvascular endothelial cells (LMVEC) was cocultured with rat pulmonary arterial smooth muscle cells (PASMC), and the cultures were randomly divided into 4 groups: normal homotypic group (NH), normal coculture group (NC), lipopolysaccharide homotypic group (LH), LPS coculture group (LC). The cell cycles were analysed with flow cytometry. The results showed that after the treatment of 100ng/ml LPS for 16h, the number of cells in G 1 phase was decreased in both homotypic and coculture of LMVEC and PASMC, while that in S+G 2/M were increased. The number of cells in G 1 phase was larger in LC than LH, but the number of cells at S+G 2/M phase was less in LC than LH. Compared with normal group, the unmber of LMVEC at S phase was increased by homotypic and coculture after the treatment with LPS. It is concluded that LPS could effect the cell cycle and accelerate the cell division, and there is a complicated regulatory mechanism in cell proliferation between LMVEC and PASMC.