大鼠不稳定膀胱与毒蕈碱受体亚型密度关系的实验研究

RELATIONSHIP BETWEEN DENSITY OF MUSCARINIC RECEPTOR SUBTYPE AND BLADDER INSTABILITY IN RATS

为了探讨毒蕈碱受体 (MR)亚型密度变化与不稳定膀胱发生之间的关系 ,建立不稳定膀胱大鼠模型 ,采用RT PCR方法测定不稳定膀胱及对照组逼尿肌MR亚型密度。结果显示 ,对照组和不稳定膀胱组逼尿肌组织均含有m2 RNA和m3 RNA ,没有检测到m1、m2 和m3 亚型的mRNA。正常对照组膀胱逼尿肌m2 RNA含量 (1 6 7± 0 4 2 )明显高于m3 RNA(0 6 4± 0 19) ,两者之比约 2 5 9∶1;不稳定膀胱逼尿肌中m2 和m3 受体密度均增加 ,分别为 (2 0 3± 0 6 5 )和(1 5 3± 0 4 6 ) ,m3 增加更明显(P <0 0 1) ,两者比值显著缩小 ,约 1 4∶1。提示不稳定膀胱的产生与MR亚型上调及M2 R、M3

To study the relationship between the density of muscarinic receptor (MR) subtype and the occurrence of bladder instability. The rat model of unstable bladder was established, and the density of MR subtype was determined in normal and unstable bladders. The results showed that there were only m 2 RNA and m 3 RNA present in the detrusor of normal and unstable bladders, and m 1, m 2 or m 3 mRNA subtypes were not detected. In the normal bladder, the amount of m 2 RNA (1 67±0 42) was obviously greater than m 3 RNA (0 64±0 19), and the ratio of them was 2 59∶1. In the unstable bladder, the density of both m 2 and m 3 increased to (2 03±0 65) and (1 53±0 46) respectively, and the increase of m 3 was more significant ( P <0 01), lowering the ratio between them to about 1 4∶1. These findings suggested that up regulation of MR subtypes and the ratio imbalance between MR subtypes were closely related to the occurrence of unstable bladder.