异丙酚对脑创伤家兔血及脑脊液神经元特异性烯醇化酶含量的影响

INFLUENCE OF POPOFOL ON THE CONTENTS OF NEURON-SPECIFIC ENOLASE IN SERUM AND CEREBROSPINAL FLUID OF RABBITS WITH BRAIN INJURY

通过观察异丙酚对脑创伤家兔血及脑脊液神经元特异性烯醇酶 (NSE)含量的影响 ,探讨异丙酚的脑保护作用。用 2 0只健康雄性新西兰家兔建立稳定的脑创伤模型 ,随机分为对照组和异丙酚组 (n=10 ) ,分别于伤前 ,伤后 4、2 4、4 8、72h和 1周测定血和脑脊液NSE含量。结果显示 ,对照组血NSE含量于伤后 2 4、4 8、72h ,脑脊液NSE含量于伤后 4、2 4、4 8、72h和 1周明显高于伤前 (P <0 0 5 ,P <0 0 1) ;异丙酚组血NSE在伤后 4 8、72h和 1周 ,脑脊液NSE含量在伤后 2 4、4 8、72h和 1周明显低于对照组 (P <0 0 5 )。表明异丙酚能明显降低脑创伤家兔血和脑脊液NSE含量 。

To observe the effect of propofol on the change in neuron specific enolase(NSE) contents in the serum and cererospinal fluid(CSF) of rabbits suffering from brain injury, so as to explore the protective effect of propofol on the brain, 20 New Zealand rabbits were inflicted with brain injury and randomized into control group and propofol treatment group ( n =10 each). Samples of serum and CSF were collected before trauma and 4h, 24h, 48h, 72h and one week following trauma respectively. The contents of NSE in serum and CSF were measured by enzyme immunoassay (EIA) method. The results showed that the content of NSE in serum at 24h, 48h, 72h and one week, and that in CSF from 4h to one week after trauma in control group were elevated significantly as compared with those before trauma ( P <0 05, P <0 01). The content of NSE in serum at 48h, 72h and one week, and that in CSF at 24h, 48h, 72h and one week posttrama in the group treated with popofol were lower than those in control group ( P <0 05). Propofol might have a protective effect on traumatic brain injury as shown by a significant decrease in the content of NSE in serum and CSF.