人类唾液粘蛋白对口腔变形链球菌凝集的实验研究

The effect of human high-molecular-weight salivary mucin on the aggregation of streptococcus mutans.

目的 :观察人的腮腺唾液 (PARS)、除腮腺外唾液 (EPS)及纯化的高相对分子质量唾液粘蛋白 (MG1)对变形链球菌Ingbritt、LM 7、远缘链球菌OMZ 176的凝集作用。方法 :采用分光光度计 (酶标仪 )测其A570值 ,测定唾液及MG1对变链菌和远缘链球菌的凝集力。结果 :EPS介导的凝集力最高 ,MG1次之 ,PARS最低。结论 :MG1具有一定介导口腔变链菌凝集作用 ,但凝集力低于EPS 。

0bjective: To observe the effects of parotid saliva(PARS), extraparotid saliva(EPS) and purified high molecular weight mucin(MG1) on the aggregation of streptococcus mutans . Methods: PARS, EPS and MG1 were prepared routinely and applied in the cultures of S. mutans Inbritt, S.mutans LM and S.sobrinus OMZ 176 respectively for 2 h. The aggregation of the bacteria was measured spectrophotometrically.Results: The aggregation (%) of S.mutans Inbritt, S.mutans LM and S.sobronus OMZ 176 induced by EPS was 32.80 , 57.87 and 35.46 , that by MGI 25.68, 32.77 and 24.16 , that by PARS 13.52, 22.23 and 16.00, respectively. Conclusion: The effect of MGI on the aggregation of streptoccus mutans is weaker than that of EPS and stronger than that of PARS. The aggregation may be primarily induced by mucins.