人鼻咽上皮组织中间隙连接蛋白基因表达谱的研究

Expression of genes encoding gap junction protein in normal human nasopharyngeal epithelial tissue

目的 :研究细胞间隙连接蛋白基因 (Cx)在正常人鼻咽上皮组织中的表达谱 ,在此基础上探索细胞间隙连接蛋白在鼻咽癌组织中的表达 ,为研究鼻咽组织癌变的原因和机理提供新思路。方法 :根据基因库中人间隙连接蛋白基因的mRNA序列 ,合成间隙连接蛋白基因家族中 1 3个基因的PCR引物 ,用RT -PCR的方法检测这些基因在鼻咽组织中的表达。结果 :1 8例正常人鼻咽组织中 ,检出Cx30、31 .1、37、40、43分别有 1 6、1 6、1 7、1 5、1 7例表达 ,Cx2 6、31、32、45分别有 1 0、1 1、9、9例表达 ,Cx36、46、46 .6、50分别有 1、0、1、3例表达。结论 :正常人鼻咽组织中 ,主要表达的间隙连接蛋白为Cx30、31 .1、37、40。

AIM: To examine the expression of gap junction protein family genes, including thirteen independent genes, in normal human nasophryngeal epithelial tissue and to conjecture the possible roles of gap junction proteins in nasopharyngeal carcinoma. METHODS: With synthesized primers, the expression of thirteen genes encoding different gap junction proteins in human normal nasopharyngeal epithelial tissue were detected by RT-PCR. RESULTS: In 18 samples of normal human nasopharyngeal epithelial tissue, 16 of them were found the expression of Cx 30, 31 1, 17 of them were found the expression of Cx 37 and Cx 43, and Cx 40 expression were detected in 15 samples. Also the expression of Cx 26, 31, 32, 36, 45, 46, 46 6, 50 were detected respectively in 10, 11, 9, 1, 9, 0, 1,3 samples of the 18 cases. CONCLUSION: In normal human nasopharyngeal tissue, Cx 30, 31, 31 1,37, 40, 43 might be the key gap junction proteins.