大网膜防御机制的研究

Defense mechanism of the greater omentum

目的 :通过观察大鼠大网膜乳斑的核苷酸酶 (5’ -nucleotidase )活性及淋巴管的定位 ,从一个新的角度来探讨大网膜的防御机制。方法 :采用大鼠大网膜全载标本 ,进行酶组织化学、免疫组织化学染色 ,光学显微镜和共焦点激光荧光显微镜观察。结果 :乳斑深层淋巴细胞呈 5’ -nucleotidase强阳性 ;乳斑表层脂肪细胞间隙发现大量的无基底膜的毛细淋巴管。结论 :结果提示乳斑淋巴细胞可中介腺苷抑制自身或其他乳斑免疫细胞的活动 ;

AIM:To observe the distribution of 5'-nucleotidase positive of milky spot and lymphatic vessels in the greater omentum and investigate the defense mechanisms of the greater omentum in another way. METHODS:The whole mount preparations of the greater omentum was stained with enzyme histochemistrical and immunohistochemistrical methods and observed with light microscopy and confocal laser scanning microscopy. RESULTS: Positive reaction was showed in lymphocytes at deep portions of the milky spot. A lot of lymphatic capillaries which had no basement membrane were found in intercellular spaces of adipose cells at superficial portions of the milky spot. CONCLUSION: These findings indicated that the adenosine formed in the milky spot inhibited the action of immunocyte in the milky spot. The lymphatic capillaries in the superficial portions may play some roles with the defense mechanisms of the greater omentum.