摘要
目的:研究抑制枯否细胞对冷保存的鼠肝枯否细胞和肝细胞线粒体的影响。方法:实验组连续2 d静注氯化钆,7 mg/kg,对照组静注等量生理盐水。3 d后手术取肝,保存时间分别为1,2,3和4 h。观察各组枯否细胞和肝细胞线粒体的形态变化,测定肝组织和肝细胞线粒体脂质过氧化物(LPO)的含量。结果:对照组枯否细胞表现为细胞膜突起增多,细胞内颗粒物质增多等。肝细胞线粒体普遍肿胀,内膜嵴断裂,空泡形成以及破坏等。实验组肝组织和肝细胞线粒体脂质过氧化程度在冷保存1 h和2 h显著低于对照组(P<0.05)。结论:抑制枯否细胞对鼠肝的冷保存损伤有一定的保护作用。
Objective; Our aim was to study the effects on Kupffer cell and hepatocellular mitocondria by the inhibition of Kupffer cell. Methods: The rats in the experimental group were pretreated with gadolinium(7 mg/kg) by intro-veinal injection for 2 days, and the rats in the control group were treated with saline solution. The livers of rats were cut off by operation and preserved in the saline at 0 to 4℃for 1, 2, 3, and 4 hours. The changes of ultrastructure of Kupffer cell and hepatocellular mitochondria were observed, and the contents of lipid peroxide (LPO) in hepatic tissue and hepatoceliu-lar mitochondria were determined. Results: Ultrastructural observation showed that the processes of cellular membrane and granular substance in Kupffer cell increased in the control. Swelling,break of the ridge of inner membrane, formation of bubbles and damage in mitochondria could be seen. In the experiment group, the content of LPO of liver tissues and mitochondria in cold preservation for 1 and 2 hours reduced extraordinarily compared with the control ( P < 0. 05 ). Conclusion: Inhibiting kupffer cell can alleviate the injury of lipid peroxide in livers of rats during cold preservation.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2003年第1期9-12,共4页
Journal of China Medical University
基金
辽宁省自然科学基金课题(619025)
关键词
肝保存
枯否细胞
线粒体
脂质过氧化
大鼠
liver preservation
Kupffer cell
mitochondria
lipid peroxide
rat