抑制枯否细胞可以减轻鼠肝在冷保存中的脂质过氧化损伤

Inhibiting Kupffer Cell in Alleviating Injury of Lipid Peroxide in Liver of Rat During Cold Preservation

目的:研究抑制枯否细胞对冷保存的鼠肝枯否细胞和肝细胞线粒体的影响。方法:实验组连续2 d静注氯化钆,7 mg/kg,对照组静注等量生理盐水。3 d后手术取肝,保存时间分别为1,2,3和4 h。观察各组枯否细胞和肝细胞线粒体的形态变化,测定肝组织和肝细胞线粒体脂质过氧化物(LPO)的含量。结果:对照组枯否细胞表现为细胞膜突起增多,细胞内颗粒物质增多等。肝细胞线粒体普遍肿胀,内膜嵴断裂,空泡形成以及破坏等。实验组肝组织和肝细胞线粒体脂质过氧化程度在冷保存1 h和2 h显著低于对照组(P<0.05)。结论:抑制枯否细胞对鼠肝的冷保存损伤有一定的保护作用。

Objective; Our aim was to study the effects on Kupffer cell and hepatocellular mitocondria by the inhibition of Kupffer cell. Methods: The rats in the experimental group were pretreated with gadolinium(7 mg/kg) by intro-veinal injection for 2 days, and the rats in the control group were treated with saline solution. The livers of rats were cut off by operation and preserved in the saline at 0 to 4℃for 1, 2, 3, and 4 hours. The changes of ultrastructure of Kupffer cell and hepatocellular mitochondria were observed, and the contents of lipid peroxide (LPO) in hepatic tissue and hepatoceliu-lar mitochondria were determined. Results: Ultrastructural observation showed that the processes of cellular membrane and granular substance in Kupffer cell increased in the control. Swelling,break of the ridge of inner membrane, formation of bubbles and damage in mitochondria could be seen. In the experiment group, the content of LPO of liver tissues and mitochondria in cold preservation for 1 and 2 hours reduced extraordinarily compared with the control ( P < 0. 05 ). Conclusion: Inhibiting kupffer cell can alleviate the injury of lipid peroxide in livers of rats during cold preservation.