小儿急性白血病外周血中单个核细胞IL-8及受体mRNA表达的研究

Expression of Interleukin-8 and Its Receptor mRNA in Peripheral Blood of Acute Leukemia

目的:单个核细胞白细胞介素-8(IL-8)及其受体(IL-8R)基因mRNA在小儿急性白血病(CAL)的表达。方法:应用RT-PCR技术对CAL的外周血做IL-8及IL-8R mRNA检测。结果:CALIL-8及IL-8R水平均显著高于正常人。初发CAL IL-8 mRNA 78.13％;化疗后CAL mRNA 31.58％表达。初发CAL IL-8R mRNA 84.36％表达;化疗后mRNA36.84％表达。结论:IL-8及IL-8R基因mRNA在CAL的初发中有明显表达,在化疗后表达不明显。

Objective: Our aim was to investigate the expression of interleukin-8 (IL-8) and its receptor (IL-8R) gene mRNA in the peripheral blood of child acute leukemia (GAL). Methods:The IL-8 and IL-8R mRNA were detected by using RT-PCR in the peripheral blood samples of CAL. Results:The expression levels of IL-8 and 1L-8R of CAL were significantly higher than those of normal children. The positive expression rate of IL-8 mRNA in incipient peripheral blood samples of CAL was 78. 13% , while in post-chemotherapeutic samples the positive rate was 31.58%. Similarly, the positive expression rate of IL-8R mRNA in incipient samples of CAL was 84. 36% , while only 36. 84% in the post-chemotherapeutic samples. Conclusion: The extensive expressions of IL-8 and IL-8R mRNA could be detected in incipient patients of CAL,while in those who had undergone chemotherapy,the genes of IL-8 and IL-8R did not express significantly.