格林-巴利综合征患者的GM1抗体与空肠弯曲菌感染的关系

Antibodies to Ganglioside GM1 and Campylobacter Jejuni in the Sera of Guillain-Barre Syndrome Patients

目的 检测GBS患者血清中的GM1抗体 ,并且观察其与CJ抗体的关系 ,以试图发现CJ是否是通过GM 1样结构致敏导致GBS的发生。方法 用间接ELISA法在 81例GBS患者、34例其他神经疾病患者 (OND)和 6 3例正常对照 (NC)的血清测定了CJ抗体和GM 1抗体。结果 GBS组IgM型抗体和IgG型抗体的阳性率分别为 30 .9%和 34.6 % ,与OND组和NC组差异均有显著性。在IgM型GM 1抗体阳性的GBS患者 6 8%有CJ近期感染 ,IgG型GM1抗体阳性的患者 5 3.6 %有CJ近期感染。IgM型GM 1抗体与IgM型CJ抗体和CJ近期感染有关 ,而IgG型GM1抗体与IgG型CJ抗体和CJ近期感染无关。结论 IgM型GM 1抗体与CJ近期感染有关 ,而IgG型GM 1抗体与CJ近期感染无关。CJ感染后可通过GM1样结构发生交叉反应导致神经组织结构和功能的改变 。

Objective To test whether CJ induces GBS via GM1-like structure by detecting GM1 antibody and CJ antibody in the serum of Guillain-Barre syndrome patients. Methods Eighty-one GBS patients, 34 with other neurological diseases (OND) and 63 healthy controls (NC) were tested for GM1 antibody and CJ antibody by indirect ELISA method with ganglioside GM1 and purified CJ antigens. Results In GBS group, GM1 IgM antibody was 30.9% and GM1 IgG antibody was 34.6%. There were differences in both antibody isotypes between GBS and OND groups, as well as GBS and NC groups. 68.0% and 53.6% of the GBS patients with GM1 IgM and GM1 IgG antibodies had recent CJ infection respectively. There was correlation between GM1 IgM antibody and recent CJ infection, while no correlation for GM1 IgG antibody. There was correlation between GM1 IgM antibody and CJ infection and CJ IgM antibody, while no correlation between GM1 IgG antibodies and CJ infection or IgG antibodies. Conclusions CJ may induce GBS via GM1-like structure and GM1 antibody, but not all GM1 antibodies derive from CJ infection.