浙南地区脑血管病与载脂蛋白E基因型关系分析

Apolipoprotein E Gene Polymorphism in People with Cerebrovascular Disease in South of Zhejiang Province

目的 探讨浙南地区脑血管病患者与载脂蛋白E(apoE)基因多态性的关系。 方法 将脑血管病患者分为脑出血组与脑梗死组和对照组 ,分别以聚合酶链反应限制性片段长度分析法 (PCR -RFLP)检测ApoE基因型。结果 对照组ApoE基因型以ε3 3最多 ,2组脑血管病患者也以ε3 3最多 ,其次为ε3 4。脑梗死组ε3 4基因型、ε4等位基因频率均显著高于对照组 (P <0 .0 5 ) ;脑出血组则ε2、ε4等位基因频率略高于对照组 ,但差别无统计学意义 (P >0 .0 5 )。结论 ApoEε基因多态性与浙南地区脑血管病患者关系密切 ,ε4基因型可能是脑梗死的危险因素之一 ,但与脑出血之间尚无明确的关系。

Objective To investigate the relationship between polymorphism of apolipoprotein (apo) E gene and cerebrovascular disease patients in south Zhejiang province. Methods Eighty patients of cerebrovasacular disease, 40 for cerebral infarction and 40 for cerebral hemorrhage, and 60 normal controls were included. Polymerase chain reaction (PCR), Hha I digestion, polyacrylamide elelectrophoresis, and silver staining were used for the detection of apo E genotype. Results The results showed that the apo E ε3/3 genotype frequency and ε3 allele frequency were highest in all of the three groups. But the apo E ε3/4 genotype frequency and ε4 allele frequency in patients with cerebral infarction were higher than that of normal controls. And no difference of apolipoprotein E genetype was found between cerebral hemorrhage and the normal control groups. Conclusions The results suggested that apo E allele could be considered as a risk factor for the onset of cerebral infarction in people of south Zhejiang province. But no evidence of relationship was found in apoliprprotein E genetype and cerebral hemorrhage.