期刊文献+

一氧化氮合成酶在膀胱癌中的表达及意义 被引量:2

Expression of NOS in bladder transitional cell carcinoma
原文传递
导出
摘要 目的 了解三种类型一氧化氮合成酶 (NOS)在膀胱癌中的表达情况 ,探讨其与肿瘤发生发展的关系。 方法 对 2 5例开放手术切除的膀胱癌组织标本进行三种NOS免疫组织化学染色 ,自动图像分析仪对染色程度分级 ;6例肾移植供体正常膀胱组织标本作为对照。 结果 诱导型NOS(iNOS)在肿瘤上皮细胞呈阳性表达 ,在正常膀胱移行细胞不表达或仅微弱表达 (P <0 .0 5)。内皮细胞型NOS (eNOS)在肿瘤基质毛细血管内皮细胞中的表达阳性率 1 0 0 % ,高于对照组中的 67%。两组标本中神经型NOS(nNOS)表达部位及强度相似。统计学分析显示iNOS和eNOS表达与肿瘤分级、分期无明显相关性 ,P均 >0 .0 5。 结论 iNOS及eNOS在膀胱癌的高表达可能与肿瘤的发生发展有关。 Objective To evaluate the role of different kinds of nitric oxide synthase(NOS)in the growth and angiogenesis of bladder transitional cell carcinoma. Methods Bladder transitional cell carcinoma tissue specimens were procured from 25 patients undergoing cystectomy, and compared with 6 normal bladder tissue specimens from donors in renal transplantation. Immunohistochemical staining was performed to detect the expression of 3 kinds of NOS. Results The malignant epithelial cells showed strong positive on immunohistochemistry whereas normal bladder showed only a weak positive iNOS immunostaining. The endothelial cells of precapillary vessels in the stroma of carcinoma showed a highly positive endothelial NOS (eNOS) immunostaining as compared with the stroma of normal bladder tissue. The expression of neuronal NOS(nNOS) was found in fibers in the fibromuscular stroma of the two groups. Conclusions Bladder transitional carcinoma tissue had a higher content of iNOS and which might be related to the genesis and development of bladder carcinoma.
出处 《中华泌尿外科杂志》 CAS CSCD 北大核心 2003年第1期28-30,共3页 Chinese Journal of Urology
关键词 一氧化氮合成酶 膀胱癌 基因表达 临床意义 免疫组织化学 Bladder neoplasms Nitric oxide synthase Carcinoma,transitional cell
  • 引文网络
  • 相关文献

参考文献1

共引文献16

同被引文献17

  • 1李伟东,黄桂晓.浅表性膀胱癌组织中肿瘤抑制因子Maspin的表达研究[J].深圳中西医结合杂志,2007,17(2):87-88. 被引量:1
  • 2SHOCHINA M,FELLIG Y,SUGHAYER M,et al.Nitric oxide synthase immunoreactivity in human bladder carcinoma[J].Mol Pathol,2001,248 (54):248-252.
  • 3JENKINS DC,CHARLES IG,THONSEN LL,et al.Roles of nitric oxide in tumor growth[J].Prol Natl Acad Sci USA,1995,92 (2):4392-4396.
  • 4LIN Z,CHEN S,YE C,et al.Nitric oxide synthase expression in human bladder cancer and its relation to angiogenesis[J].Urol Res,2003,31(4):232-235.
  • 5BEECKEN WD,ENGL T,ENGELS K,et al.Clinical relevance of maspin expression in bladder cancer[J].World J Urol.2006,24(3):338-344.
  • 6FRIEDRICHA MG,TOMAA MI,HULAND H,et al.Expression of maspin in non-muscle invasive bladder carcinoma:correlation with tumor angiogenesis and prognosis[J].Eur Urol,2004,45:737-743.
  • 7KHALKHALI-ELLIS Z,HENDRIX MJ.Nitric oxide regulation of maspin expression in normal mammary epithelial and breast cancer cells[J].Am J Pathol,2003,162:1411-1417.
  • 8GIRISH GV,BHATTACHARYA G,SINHA AK.The role of insulin dependent NO synthesis in the impaired production of maspin in human breast cancer[J].J Cancer Res Clin Oncol,2006,132(6):389-398.
  • 9Jenkins DC,Charles IG,Thonsen LL,et al. Roles of nitric oxide in tumor growth [J]. Prol Natl Aead Sci USA, 1995, 92 (2):4 392-4 396.
  • 10Parenti A, Morbldellil L, Cui XL. Nitric oxide is a upstream signal of vascular endothelial growth factor-induced extracellular signal-regulated kinase1/2 activation in postcapillary endothelium[ J]. J Biol Chem,1998,273(5) :4 220-4 226.

引证文献2

相关主题

;
使用帮助 返回顶部