摘要
目的 了解三种类型一氧化氮合成酶 (NOS)在膀胱癌中的表达情况 ,探讨其与肿瘤发生发展的关系。 方法 对 2 5例开放手术切除的膀胱癌组织标本进行三种NOS免疫组织化学染色 ,自动图像分析仪对染色程度分级 ;6例肾移植供体正常膀胱组织标本作为对照。 结果 诱导型NOS(iNOS)在肿瘤上皮细胞呈阳性表达 ,在正常膀胱移行细胞不表达或仅微弱表达 (P <0 .0 5)。内皮细胞型NOS (eNOS)在肿瘤基质毛细血管内皮细胞中的表达阳性率 1 0 0 % ,高于对照组中的 67%。两组标本中神经型NOS(nNOS)表达部位及强度相似。统计学分析显示iNOS和eNOS表达与肿瘤分级、分期无明显相关性 ,P均 >0 .0 5。 结论 iNOS及eNOS在膀胱癌的高表达可能与肿瘤的发生发展有关。
Objective To evaluate the role of different kinds of nitric oxide synthase(NOS)in the growth and angiogenesis of bladder transitional cell carcinoma. Methods Bladder transitional cell carcinoma tissue specimens were procured from 25 patients undergoing cystectomy, and compared with 6 normal bladder tissue specimens from donors in renal transplantation. Immunohistochemical staining was performed to detect the expression of 3 kinds of NOS. Results The malignant epithelial cells showed strong positive on immunohistochemistry whereas normal bladder showed only a weak positive iNOS immunostaining. The endothelial cells of precapillary vessels in the stroma of carcinoma showed a highly positive endothelial NOS (eNOS) immunostaining as compared with the stroma of normal bladder tissue. The expression of neuronal NOS(nNOS) was found in fibers in the fibromuscular stroma of the two groups. Conclusions Bladder transitional carcinoma tissue had a higher content of iNOS and which might be related to the genesis and development of bladder carcinoma.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2003年第1期28-30,共3页
Chinese Journal of Urology