人类主要组织相容抗原DR分子在口腔鳞癌中表达的研究

Human Leukocyte Antigen-DR Expression and Transcription in Oral Squamous Cell Carcinomas

目的 从基因转录水平和蛋白表达水平探讨了人主要组织相容抗原DR(HLA_DR)抗原分子在口腔鳞癌演变过程中的表达改变。方法 应用HLA_DR特异性单抗和HLA_DRβ基因特异性寡核苷酸探针分别通过免疫组织化学及原位杂交的方法分别从蛋白及转录水平检测了 32例人口腔鳞癌原发灶、15例颈淋巴结转移灶和 2 6例正常口腔粘膜上皮标本内HLA_DR的表达。结果 口腔鳞癌原发灶内HLA_DR的表达显著性的高于正常口腔粘膜组 (P <0 0 5 ) ;淋巴结转移灶的HLA_DR表达与原发灶和正常粘膜均未见统计学差异。原位杂交和免疫组化对于HLA_DR表达的检测具有一致性。结论 HLA_DR在口腔鳞癌中存在有表达异常增高的现象 。

Objective\ The purpose of this study was to investigate the transcription and expression levels of human leukocyte antigen_DR at different stages of oral squamous cell carcinomas.Methods\ The specific monoclonal antibody and β_locus specific oligonucleotide probes of human leukocyte antigen_DR were employed in this study. A total of 32 primary oral squamous cell carcinomas, 15 metastatic focuses and 26 histologically normal oral epithelia, were detected for the presence of the human leukocyte antigen_DR molecule by using immunohistochemistry and in situ hybridization.Results\ The human leukocyte antigen_DR expression of the primary focuses was significantly higher than that of the normal epithelia (P<0.05), but their expression did not show statistically difference between the metastatic focuses and the normal epithelia. The immunohistochemistric results were identical with those of in situ hybridization.Conclusion\ The abnormal higher expression of the HLA_DR is a common character of primary oral squamous cell carcinomas, but it may be not relevant to the metastasis of tumors.\;