氯奎减缓过氧化氢自分解作用的检测

Inhibitive Effects of Chloroqquine on the Decomposition of Hydrogen Peroxide

目的 检测和验证氯奎 (CQ)对过氧化氢 (HP)自分解作用的抑制效果。方法 依据辣根过氧化物酶与底物 (OPD_HP)反应发生颜色改变原理 ,将以HP为主要成分的色素牙漂白剂分成 4组 ,分别加入不同剂量的CQ ,应用灵敏的酶联免疫实验方法 ,对HP的稳定性进行了 3个月的追踪检测。结果 4组制剂随贮存时间的延长 ,其吸光值都降低 ,但程度不同 ,和CQ的含量呈依赖关系。CQ含量越高其吸光值越大 ,最高浓度与对照组比较 ,高 4～ 6倍。结论 CQ具有抑制HP自分解的作用。用含HP的漂白剂治疗色素牙时 ,加入适量CQ ,不但漂白效果好 。

Objective\ The purposes of this study were to investigate effects of chloroqquine (CQ) on inhibiting the decomposition of hydrogen peroxides (HP), and to optimize composition of the tooth_ bleaching agent.Methods\ According to the principle of the color_changing reaction between horse radish peroxidase and substrate, the tooth_bleaching agent made of HP was divided into four groups with different amounts of CQ. The stability of HP was observed using ELISA for 3 months.Results\ With the prolongation of store time, the absorbance of HP solutions containing different concentrations of CQ declined at different degrees, which showed a dependent relation between absorbance values and concentrations of CQ. Within the range of experiment concentrations of CQ, the higher the concentration of CQ was, the stronger the stability of HP was. And the duration of tooth_bleaching effects in 150.0 mg/ml of CQ was prolonged 4 to 6 times compared to that without CQ.Conclusion\ The data indicate that CQ can inhibit the decomposition of HP. The bleaching effect of the tooth_bleaching agent which is made of HP and proper amount of CQ is satisfactory.\;