兔颞下颌关节腔穿刺定位及滑液抽取方法的研究

A Study on Puncturing the Temporomandibular Joint Cavity of Rabbits and Withdrawing Synovia

目的 对比各种对兔颞下颌关节腔的穿刺定位以及关节滑液提取的方法。方法 12只新西兰白兔双侧颞下颌关节分别按传统穿刺、改良穿刺、X线引导下穿刺、彩色超声诊断仪引导下穿刺、螺旋CT引导下穿刺以及运用微泵进行滑液的抽取进行对比实验。结果 传统穿刺法仅 1个关节腔成功注射 ,且未抽到滑液 ;改良穿刺法 4个关节腔均成功注射但未抽到滑液 ;X线及彩色超声均定位不准确且不能抽到滑液 ;螺旋CT可直观动态观察且定位准确但也未抽到滑液 ;微泵可以抽取滑液。结论 运用改良穿刺法及螺旋CT可以准确的行兔颞下颌关节腔穿刺 。

Objective\ The purpose of this study was to study the methods of how to puncture the temporomandibular joint (TMJ) cavity of rabbits and get the synovia.Methods\ The anthors punctured joint cavity and withdrew the synovia from bilateral TMJ cavities of 12 New Zealand rabbits with different methods, such as the traditional puncture method, improved puncture, puncture under X ray, puncture guided by ultrasonography and spiral CT, and withdrawing synovia by micropump.Results\ Only one cavity was successfully punctured using the traditional puncture method. A total of 4 cases were successfully punctured using the improved method. X ray and ultrasonography did not locate the TMJ cavity precisely. The spiral CT helped observation and puncture. However, the above methods did not work in collecting synovia. The method of micropump withdrew synovia successfully.Conclusion\ The improved method and spiral CT are efficient methods to precisely puncture TMJ cavity. The technique of micropump successfully resolves the problem of withdrawing synovia.\;