急性胰腺炎与胰腺腺泡细胞凋亡的关系

A study on the relationship between acute pancreatitis and apoptosis of the acinar cells

目的研究急性胰腺炎严重程度与胰腺腺泡细胞凋亡的关系。方法建立不同严重程度的急性胰腺炎大鼠模型 ,通过原位末端标记法定量检测胰腺腺泡细胞凋亡率。结果单纯水肿性胰腺炎时极少有腺泡细胞凋亡。出血坏死性胰腺炎时有腺泡细胞凋亡 ,且随着出血坏死程度的加重 ,腺泡细胞的凋亡先较快地增加后逐渐减少 ,细胞坏死则逐渐增多。结论在急性单纯水肿性胰腺炎时极少有腺泡细胞凋亡。急性胰腺炎的严重程度与腺泡细胞的凋亡率在程度较轻的急性出血坏死性胰腺炎时呈正相关 ,而在程度较重的急性出血坏死性胰腺炎时则呈负相关。

ObjectiveTo investigate the relationship between the severity of acute pancreatitis and apoptosis of acinar cells. MethodsThe apoptotic ratio of acinar cells was measured in rat acute pancreatitis model by methods of in situ end labeling.ResultsThere was almost no apoptotic acinar cells in the model of acute edematous pancreatitis. With the increase in the severity of the pancreatitis apoptotic cells were more and more common until to the late stage of hemorrhagic and necrotic pancreatitis when the apoptotic ratio of acinar cells dwindled, meanwhile the necrotic acinar cell increased continuously.ConclusionsIn acute simple edematous pancreatitis apoptotic acinar cells is infrequently seen. In acute moderate hemorrhagic and necrotic pancreatitis the ratio is correlated positively with the severity of pancreatitis.In the late stage the ratio was correlated negatively with the severity of pancreatitis.