摘要
Ribes plants,like most of other fruit trees,are characterized by their large number but small size of somatic chromosomes.In the light of these special characteristics,we have examined several factorial combinations of material, pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants. The resuts indicated that the combination of root tip with pretreatment of 2 m molL-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3～5 hours at 14℃,pre-hypotonic trcarment of 0.07mol L-1 KCl for 30 min, fixation in Carnoy's fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4～5 hours at 25℃, post-hypotonic treatment in distilled water for half an hour and staining in Giemsa could make the chromosome prepa ration superior to other treatment combinations.
Ribes plants,like most of other fruit trees,are characterized by their large number but small size of somatic chromosomes.In the light of these special characteristics,we have examined several factorial combinations of material, pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants. The resuts indicated that the combination of root tip with pretreatment of 2 m molL-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3～5 hours at 14℃,pre-hypotonic trcarment of 0.07mol L-1 KCl for 30 min, fixation in Carnoy's fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4～5 hours at 25℃, post-hypotonic treatment in distilled water for half an hour and staining in Giemsa could make the chromosome prepa ration superior to other treatment combinations.
