军曹鱼不同组织5种同工酶的研究

The Isozyme Analysis of Different Tissue in Rachycentron canadum

采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织 (脑、心肌、肾、肌肉、性腺、肝、血液、胰、脾 )的 5种同工酶 (LDH、MDH、ME、POD和EST)进行了分析 ,结果表明 :5种同工酶在军曹鱼中都具有明显的组织特异性 ,这些特异性与其组织特定生理功能相关。与其他硬骨鱼类相比 ,军曹鱼的酶谱简单得多 ,这可能与其种属多样性相关。

By the vertical polyacrylamide gel electrophoresis,five isozymes (LDH,MDH,ME, POD,EST) from several tissues(brain, heart, muscle, kidney, gonad, liver, blood, spleen, pancreas) of R achycentron canadum were studied, the results showed that all isozymes presented tissue specificity. These specificities were related to their special physiological function. Compared with other teleosts, the isozymic phenotypes of R. canadum were much simple with, which may be related to its generic diversity.