辐照木霉菌株的生物学效应研究

BIOLOGICAL EFFECT OF 60 Co γ IRRADIATION ON Trichoderma

本文研究了6 0 Coγ射线辐照木霉 (Trichodermaspp)菌株的诱变效果。结果表明 :在γ射线的作用下 ,木霉分生孢子萌发率显著降低 ,孢子萌发时间加长 ,部分萌发孢子菌丝生长畸形 ;随着照射剂量的增加 ,木霉孢子存活率急剧下降 ,剂量效应曲线符合一次击中模型S =e-λD;诱变效应方程为 :Y =-0 940e1 2 9x,r=0 953 3 (r0 0 1=0 765)。依各菌株对生长速度、产孢量、开始产孢时间、菌落颜色等变异情况 ,初筛得到变异菌株 1 3 0株。

The mutagenetic effect of 60 Co γ\|ray on Trichoderma spp (T 32 ) was studied.The germination capacity of spores apparently declined after irradiation.The mutagenetic effect curve model is S=e -λD ,and the mutagenetic curve is Y =-0 940e 1 29x , r =0 9533( r 0 01 =0 765). 130 variant strain which grew rapidly and produced more spores were chosen.