大豆黄酮对卵巢切除大鼠鼻黏膜上皮细胞凋亡的保护作用

Protective effect of daizein on apoptosis cells of nasal mucosas in ovariectomized rats

目的 观察双侧卵巢切除诱导大鼠鼻黏膜上皮细胞凋亡 ,研究尼尔雌醇、大豆黄酮对此的保护作用。方法 将 60只大鼠随机分为 4组 :健康对照组 ,卵巢切除组 ,卵巢切除 +尼尔雌醇组 ,卵巢切除 +大豆黄酮组。各组动物分期分 3批处死 ,流式细胞仪检测各组动物鼻中隔黏膜的早期凋亡细胞。结果 双侧卵巢切除后 ,大鼠鼻黏膜的早期凋亡细胞百分数增高 ,术后 60d达到新的平衡。大豆黄酮、尼尔雌醇干预组与健康组比较 ,早期凋亡细胞无明显改变。

Objective To study the protective effect of daizein on apoptosis cells of atrophic nasal mucosas in ovariectomized rats Methods Sixty rats were divided into four groups as contrary, ovariectomized, ovariectomized+nylestriol ( O+N ) and ovariectomized+daizein (O+D), each with 15 rats Earlier apotosis cells of nasal mucosas taken from nasal septum were measured with flow cytometry Results Compared with contrary group, the number of apoptosis cells of mucosas increased after being ovariectomized, the number of apoptosis cells of mucosas in O+N and O+D groups didn′t change Conclusion Estrogen replacement and daizein might have effects on protecting cells of mucosas from lacking of estrogen by decreasing apoptosis cells in ovariectomized rats