大黄素对BXSB狼疮样小鼠肾脏病变的影响及其机制

Effect of emodin on nephritis of BXSB lupus mice and its pharmacological mechanism

目的 : 探讨大黄素治疗狼疮性肾炎的作用及其机制。方法 : 取已发生狼疮性肾炎的BXSB雌性小鼠 3 5只 ,分别予以胃饲不同浓度的大黄素混悬液和生理盐水。 3 0天后 ,检测每只小鼠的 2 4h尿蛋白含量 ,同时取出肾脏制成切片 ,SP(streptavidin/peroxidase)法检测肾小球细胞间粘附分子 1(ICAM 1)表达水平 ,并用高清晰度彩色病理图文分析系统 (HPIAS)得出定量结果。结果 : 与生理盐水组相比 ,胃饲大黄素的各组小鼠 2 4h尿蛋白含量和肾小球ICAM 1表达水平均有所下降 (P <0 .0 5 ) ,尿蛋白含量与大黄素剂量 (0～ 6.2 5mg/kg范围内 )呈直线负相关 (P <0 .0 1) ;肾小球ICAM 1表达水平与大黄素剂量呈负相关(P <0 .0 0 1) ;尿蛋白含量与ICAM 1表达水平呈正相关 (P <0 .0 0 1)。结论 : 大黄素能降低BXSB狼疮样小鼠尿蛋白水平 ;其免疫药理学机制可能与下调肾小球ICAM

Objective:To study the effect of emodin on the nephritis of BXSB lupus mice and its pharmacological mechanism.Methods:Thirty five BXSB female mice with lupus were divided into five groups, and were fed with emodin in o.9% NaCl solutionin in different dosages. Thirty days later, their proteinuria concentration was detected and expression of intercellular adhesion molecule 1 (ICAM 1) in the glomeruli was analysed by immunohistochemical method of SP (streptavidin/peroxidase) and HPIAS (high resolution pathological image analysis system). Results: Compared with the control group, the proteinuria concentration and expression of ICAM 1 in the glomeruli of the emodin groups decreased (P<0.05). The emodin dosage (0～6.25 mg/kg) showed negative correlation with the proteinuria concentration and expression of ICAM 1(P<0.001) and even a negative linear correlation with the proteinuria concentration (P<0.01). The proteinuria concentration showed a positive correlation with expression of ICAM 1 (P<0.001). Conclusion :Emodin can down regulate the proteinuria concentration of BXSB lupus mice. The possible immunopharmacological mechanism may depress ICAM 1 expression in the glomeruli.