光化学荧光分析法研究(Ⅵ)——混合色素的光化学速差动力学荧光分析法测定

Studies on the Photochemical Fluorimetry( Ⅵ)——Determination of Dye-Mixtures by Using Differential Rate Kinetic Photochemical Fluorimetry

以光化学荧光衍生化法测定苋菜红(AM)和直接耐晒嫩黄5GL(DY)2种偶氮色素,研究了同时测定这2种色素混合物的光化学速差动力学荧光分析的原理和方法。AM和DY的浓度分别在0～220ng/mL和0～120ng/mL范围内呈良好的线性关系,相关系数皆为0.999,检出限为0.64ng/mL AM和0.045ng/mL DY。相对标准偏差分别为1.6％(n=8)和1.8％(n=7)。该法用于人工合成混合样品的测定效果良好。

Photochemical fluorimetric methods for the determination of Azo-dyes, Amaranth (AM) and Direct Sun-resisting Light Yellow(DY) are described. A differential rate method is uesd for resolution of mixtures of these dyes. The principle of these methods are discussed. Linear calibration curves are obtained over the ranges of 0-220 ng/mL and 0-120 ng/mL for AM and DY, respectively (r= 0.999 for both), the limits of determination are 0.64 ng/mL AM and 0. 045 ng/mL DY. The relative standard deviation is 1. 6%(n=8) and 1. 8%(n = 7) for AM and DY, respectively, these methods have been applied to the determination of dye-mixtures with satisfactory results.